Human Reconstructed Epidermis

DESCRIPTION

The epidermis is the outer layer of the skin. The epidermis is a stratified squamous epithelium composed of proliferating basal and differentiated suprabasal keratinocytes called spinous layer, granular layer and stratum corneum.

After 17 days of tissue culture of in defined cell culture medium, epidermal keratinocytes isolated from foreskin have rebuild a human epidermal model demonstrating all the characteristics of the in vivo epidermis.

The Skin+™ reconstructed epidermis model is characterized by a high barrier function and metabolic activity and can be employed for safety and efficacy testing. To ensure global availability, models are transported on an agarose based solidified medium together with Sterlab growth and maintenance medium.

RECOMMENDED MEDIUM
Growth Medium (Ref: 205MILC – 125ml, 250ml or 500ml).

PRODUCT CHARACTERIZATION
Each production batch  is provided with quality controls values and recommendations such as storage conditions, RHE instruction for use, batch number and origin, histology, cell viability, barrier function integrity, absence of Hepatitis B, Hepatitis C, HIV, and Mycoplasma.

PRODUCT USE
The Sterlab RHE model are for in vitro research use only and not for diagnostic or clinical applications.

APPLICATIONS

The Skin+™ model can be employed to test the safety and efficacy of chemicals, cosmetics, and pharmaceutical compounds. Due to a dry surface, the Skin+ model allows realistic topical application scenarios for a broad range of liquid and solid substances. Moreover, a systemic application can be simulated by applying test substances into the culture medium.

Safety Test method

Efficacy Test method
  • Anti-oxidative capacity
  • Anti-inflammatory activity
  • Hydration
  • Anti-aging
  • Epidermal differentiation
  • Percutaneous absorption
  • Effects of UVA and UVB irradiation
  • UVB protection
  • Epidermal permeability and metabolism
  • Genomic signatures

2 résultats affichés

2 résultats affichés